ANOTHER PROOF THAT HAIRLOSS Is the problem with the REDOX and glutathione and VIRAL INFECTION

[hello]

Some scientists did. you can research it. but I think vitamin C needs to be in the active form

L-ascorbic acid in water will be fine ?

 

[hello]

@Helen Read this one http://twin.sci-hub.tw/6904/43271fdcf1a62b3b257330460d77c47a/sasaki2018.pdf

iNOS inhibits hair regeneration in obese diabetic (ob/ob) mice



Previous studies have shown that androgenic alopecia is associated with metabolic syndrome and dia- betes. However, the detailed mechanism whereby diabetes causes alopecia still remains unclear. We focused on the inflammatory response that is caused by diabetes or obesity, given that inflammation is a risk factor for hair loss. Inducible nitric oxide synthase (iNOS) is known to be upregulated under con- ditions of acute or chronic inflammation. To clarify the potential role of iNOS in diabetes-related alopecia, we generated obese diabetic iNOS-deficient (ob/ob; iNOS-KO mice). We observed that ob/ob; iNOS-KO mice were potentiated for the transition from telogen (rest phase) to anagen (growth phase) in the hair cycle compared with iNOS-proficient ob/ob mice. To determine the effect of nitric oxide (NO) on the hair cycle, we administered an iNOS inhibitor intraperitoneally (compound 1400 W, 10 mg/kg) or topically (10% aminoguanidine) in ob/ob mice. We observed that iNOS inhibitors promoted anagen transition in ob/ob mice. Next, we administered an NO donor (S-nitrosoglutathione, GSNO), to test whether NO has the telogen elongation effects. The NO donor was sufficient to induce telogen elongation in wild-type mice. Together, our data indicate that iNOS-derived NO plays a role in telogen elongation under the inflammatory conditions associated with diabetes in mice.

 

[Helen]

@Helen Read this one http://twin.sci-hub.tw/6904/43271fdcf1a62b3b257330460d77c47a/sasaki2018.pdf

iNOS inhibits hair regeneration in obese diabetic (ob/ob) mice



Previous studies have shown that androgenic alopecia is associated with metabolic syndrome and dia- betes. However, the detailed mechanism whereby diabetes causes alopecia still remains unclear. We focused on the inflammatory response that is caused by diabetes or obesity, given that inflammation is a risk factor for hair loss. Inducible nitric oxide synthase (iNOS) is known to be upregulated under con- ditions of acute or chronic inflammation. To clarify the potential role of iNOS in diabetes-related alopecia, we generated obese diabetic iNOS-deficient (ob/ob; iNOS-KO mice). We observed that ob/ob; iNOS-KO mice were potentiated for the transition from telogen (rest phase) to anagen (growth phase) in the hair cycle compared with iNOS-proficient ob/ob mice. To determine the effect of nitric oxide (NO) on the hair cycle, we administered an iNOS inhibitor intraperitoneally (compound 1400 W, 10 mg/kg) or topically (10% aminoguanidine) in ob/ob mice. We observed that iNOS inhibitors promoted anagen transition in ob/ob mice. Next, we administered an NO donor (S-nitrosoglutathione, GSNO), to test whether NO has the telogen elongation effects. The NO donor was sufficient to induce telogen elongation in wild-type mice. Together, our data indicate that iNOS-derived NO plays a role in telogen elongation under the inflammatory conditions associated with diabetes in mice.

What is here to read. I posted the same in the openning post

 

[hello]

What is here to read. I posted the same in the openning post

Confirmation of the idea dude, Good job.

What do you think , L-ascorbic acid 20% in water applied twice a day on scalp it will work ?

 

[Jkm7389]

I will save a lot of guys trouble here and say most of the benefits of urine can be had by trying out the chemical Urea. It's one of the greatest supplements with least drawbacks. No need to be drinking your own piss.

The "brain inflammation" is just excess ammonia and chemical urea works just as good(excess ammonia production by bad bacteria / slow digestion, impaired urea cycle (Citrulline, Arginine again...)

I once had this but run out Urea USP grade 8 ounce

It might be worthwhile to try out again in combination with some of the electrolytes protocol components. Would make sense if digestion, enzymes, HCL down -> Aminos, B's, minerals etc, support with Urea, Citrulline to take care of excess Ammonia allowing the systems to come up. Might be worthwhile testing out.

Hair growth by Helen also can be attributed to the Urea in urine. Here's anecdotal evidence from RPF from chemical urea:
"The topical application was to a mild wound on my forearm. Didn't really seem to heal it quickly, totally subjective opinion. But, this is also curious, several weeks later, there is a cluster of hair where the former wound was that is darker, thicker, longer, and more numerous than any other area on the forearm!"

The theory of urine having the exact individual components that produce "homeopathic effect" is tempting but I will let Helen test it out and perceive any of the changes produced over the weeks.

All roads ultimately lead to drinking urine in ALL health protocols... but it never pans out and it leaves the already desperate seeker in deeper despair.

I'm with you. I think it's all about the urea, not to mention that in your urine your body excretes excess substances it's trying to get rid of, not just waste. I guess you can't argue with results but drinking pee is just...wow. More power to you I guess if your someone who does it haha, I guess my pride will be the death of me in this case lol but pee drinking to survive is basically my idea of a dystopian future...

 

[mahounie]

I'm with you. I think it's all about the urea, not to mention that in your urine your body excretes excess substances it's trying to get rid of, not just waste. I guess you can't argue with results but drinking pee is just...wow. More power to you I guess if your someone who does it haha, I guess my pride will be the death of me in this case lol but pee drinking to survive is basically my idea of a dystopian future...

I'm not doing urine therapy, never done it. I'm considering doing it "for science" and then follow up with Urea + minerals just to compare and report the differences (hormones, unique minerals if they are good or not). I don't give a crap about the nastiness of it if it works it works, but I'm not convinced yet. I don't want to be drinking urine for 7-15+ years like some of these guys are.

 

[happenstance]

It's not nasty if it's fresh. You don't need to do it for 15 years to feel the effect, shouldn't take more than 15 minutes on an empty stomach.

 

[ballheadbuddha]

It's not nasty if it's fresh. You don't need to do it for 15 years to feel the effect, shouldn't take more than 15 minutes on an empty stomach.

Are you still doing it?
In my experience, it tastes different at different times of the day, in the morning being the strongest/most bitter, the after taste is cruel too.. I usually drinking a glass of water after it, does it change anything effect wise? should it be solely in empty stomach?
the "dirtiness" of Urine is so ingrained in our minds, that people usually doesn't even grasp the possibility of discussing about it, but it doesn't feel dirty at all after doing it for a while.

 

[happenstance]

Are you still doing it?
In my experience, it tastes different at different times of the day, in the morning being the strongest/most bitter, the after taste is cruel too.. I usually drinking a glass of water after it, does it change anything effect wise? should it be solely in empty stomach?
the "dirtiness" of Urine is so ingrained in our minds, that people usually doesn't even grasp the possibility of discussing about it, but it doesn't feel dirty at all after doing it for a while.

I had amazing results but had to stop because I became aware of some issues with acidity, and besides I've drunk some alcohol too the last days. Will start again soon. According to what I've read you should drink your urine on an empty stomach and wait at least half an hour before eating anything. Urine is basically filtered blood, I've eaten bloodsausage from pig several times.

 

[SwaZ]

Anyone else get acne like lesions on scalp? I get them on back of the head and also on the front where the temples are. Read somewhere that iNos can create these.

 

[Fester]

What do you think , L-ascorbic acid 20% in water applied twice a day on scalp it will work ?

"When A2P is systemically administered, however, A2P becomes metabolized into AA, which has no beneficial effect on the growth of dermal papilla cells."
From this paper: (PDF) Skin penetration and retention of L-Ascorbic acid 2-phosphate using multilamellar vesicles

Some studies use magnesium ascorbyl phosphate (MAP) which is readily available for cosmetic use. However it apparently does not absorb well. In another study they used MSM to increase absorption, other alternatives would be microneedling or something like a boar bristle brush. In the MSM study they used 10% MAP on its own or 7.5% MAP with 10% MSM.
(PDF) The Effect of Methylsulfonylmethane on Hair Growth Promotion of Magnesium Ascorbyl Phosphate for the Treatment of Alopecia

Would be interesting if someone who shaves or buzzes their hair tried applying this only to one side... Seems like there isn't much follow up on this in humans though, so maybe it is ineffective.

 

[hello]

"When A2P is systemically administered, however, A2P becomes metabolized into AA, which has no beneficial effect on the growth of dermal papilla cells."
From this paper: (PDF) Skin penetration and retention of L-Ascorbic acid 2-phosphate using multilamellar vesicles

Some studies use magnesium ascorbyl phosphate (MAP) which is readily available for cosmetic use. However it apparently does not absorb well. In another study they used MSM to increase absorption, other alternatives would be microneedling or something like a boar bristle brush. In the MSM study they used 10% MAP on its own or 7.5% MAP with 10% MSM.
(PDF) The Effect of Methylsulfonylmethane on Hair Growth Promotion of Magnesium Ascorbyl Phosphate for the Treatment of Alopecia

Would be interesting if someone who shaves or buzzes their hair tried applying this only to one side... Seems like there isn't much follow up on this in humans though, so maybe it is ineffective.

Alright, So I researched a bit cuz that statement about Ascorbic Acid not beneficial was very weird . Actually there is no study with such conclusion, or at least i haven't found one.

They messed up these statements big time about the fact that Ascorbic Acid is not beneficial for hair growth ,then no beneficial for fibroblast cells, then no beneficial for growth of the dermal papilla cells .

This statement is from the Abstract of the study:

"During the penetration, however, significant amount of the drug was metabolized into L-ascorbic acid, which has no beneficial effect on stimulation of hair growth. "

How on earth they got to that conclusion ? ? based on what ?

Then In the introduction of the study there are the following statements :

L-Ascorbic acid-2-phosphate magnesium salt, a phosphate derivative of L-ascorbic acid (AA), is known to stimulate growth of human dermal fibroblast cells while AA has no benefit on the growth of the cells (Hata and Senoo, 1989; Takamizawa et al.,2004).

So this statement is based on these 2 studies: (PDF) Effects of ascorbic acid and ascorbic acid 2-phosphate, a long-acting Vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells and this one : Sci-Hub | L-ascorbic acid 2-phosphate stimulates collagen accumulation, cell proliferation, and formation of a three-dimensional tissuelike substance by skin fibroblasts | 10.1002/jcp.1041380103 where you will find no such conclusion like Ascorbic acid has no benefit of growth of dermal fibroblast cells, acctualy there is this statement in the cited study:

Over a decade ago, we reported the stimulation of growth and differentiation of human dermal fibroblasts by both Asc 2-P and AsA (ascorbic acid) ( (Hata et al., 1988;Hata and Senoo, 1989).

So wrong !


Then there is second statement in the introduction :

This finding provided a potential that A2P might be useful for the treatment of androgenetic alopecia. When A2P is systemically administered, however, A2P becomes metabolized into AA, which has no beneficial effect on the growth of dermal papilla cells

Again , this statement is based on nothing and have no cited study for this statement.

This study profess wrong this statement, and actually tells us that Topically applied vitamin C increases the density of dermal papillae in aged human skin : Topically applied vitamin C increases the density of dermal papillae in aged human skin





I researched this because Ascorbic Acid is very easy to find and very cheap compared with ascorbic acid 2-phosphate.
And if works in the same way, which I think it should , could be a very nice treatment for hair-growth , cheap, easy to find and no side effects .
This is what I want to do: Create micro channels with micro-needling once a week or as soon it is healed... and apply every day at night Ascorbic Acid solution in water. Not sure yet about the dose .. I will try it for at least a month .

 

[TubZy]

Alright, So I researched a bit cuz that statement about Ascorbic Acid not beneficial was very weird . Actually there is no study with such conclusion, or at least i haven't found one.

They messed up these statements big time about the fact that Ascorbic Acid is not beneficial for hair growth ,then no beneficial for fibroblast cells, then no beneficial for growth of the dermal papilla cells .

This statement is from the Abstract of the study:
How on earth they got to that conclusion ? ? based on what ?

Then In the introduction of the study there are the following statements :



So this statement is based on these 2 studies: (PDF) Effects of ascorbic acid and ascorbic acid 2-phosphate, a long-acting Vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells and this one : Sci-Hub | L-ascorbic acid 2-phosphate stimulates collagen accumulation, cell proliferation, and formation of a three-dimensional tissuelike substance by skin fibroblasts | 10.1002/jcp.1041380103 where you will find no such conclusion like Ascorbic acid has no benefit of growth of dermal fibroblast cells, acctualy there is this statement in the cited study:
So wrong !


Then there is second statement in the introduction :

Again , this statement is based on nothing and have no cited study for this statement.

This study profess wrong this statement, and actually tells us that Topically applied vitamin C increases the density of dermal papillae in aged human skin : Topically applied vitamin C increases the density of dermal papillae in aged human skin





I researched this because Ascorbic Acid is very easy to find and very cheap compared with ascorbic acid 2-phosphate.
And if works in the same way, which I think it should , could be a very nice treatment for hair-growth , cheap, easy to find and no side effects .
This is what I want to do: Create micro channels with micro-needling once a week or as soon it is healed... and apply every day at night Ascorbic Acid solution in water. Not sure yet about the dose .. I will try it for at least a month .

What about topical sodium ascorbate? You get the salt and vit C at the same tims..both good for hair..

 

[hello]

What about topical sodium ascorbate? You get the salt and vit C at the same tims..both good for hair..

Well. i bought already the L-Ascorbic Acid .. so i am gonna go with this one.

Look at this one: Sci-Hub | The Molecular Mechanism Underlying the Proliferating and Preconditioning Effect of Vitamin C on Adipose-Derived Stem Cells | 10.1089/scd.2013.0460 , they treated Adipose-Derived Stem Cells with Ascorbic Acid then injected them in mouse skin . Hair growth was observed , and release of growth factors .

In addition, vitamin C-treated ASCs accelerated the telogen-to-anagen transition in C3H/HeN mice, and conditioned medium from vitamin C-treated ASCs increased the hair length and the Ki67-positive matrix keratinocytes in hair organ culture. Vitamin C increased the mRNA expression of HGF, IGFBP6, VEGF, bFGF, and KGF, which may mediate hair growth promotion. In summary, vitamin C is transported via SVCT2, and increased ASC pro- liferation is mediated by the MAPK pathway. In addition, vitamin C preconditioning enhanced the hair growth promoting effect of ASCs. Because vitamin C is safe and effective, it could be used to increase the yield and regenerative potential of ASCs.

 

[Potion]

Well. i bought already the L-Ascorbic Acid .. so i am gonna go with this one.

Look at this one: Sci-Hub | The Molecular Mechanism Underlying the Proliferating and Preconditioning Effect of Vitamin C on Adipose-Derived Stem Cells | 10.1089/scd.2013.0460 , they treated Adipose-Derived Stem Cells with Ascorbic Acid then injected them in mouse skin . Hair growth was observed , and release of growth factors .

You can make sodium ascorbate by dissolving ascorbic acid in water and adding sodium bicarbonate.

 

[Snow1]

Lol I can’t take this step yet either.

Yup same here, imagine trying to explain this one to your mates hahaha fark that. Thumbs up to those doing it tho.

 

[Go_faster_Sonic]

Is topical urine dangerous if i'm still on meds?

 

[MNK99]

--i doubt it. --unless the particular meds make urine very toxic or something.If pretty healthy otherwise, you'd notice that. Some people would notice that.
-I don't think it'd be useful/ideal. I don't know much about urine therapy tho.

 

[Maxin]

Drinking urea is not even close to drinking urine. If arginase is induced like in inflammation. and body is stuck in Th2 balance.you are losing into the urine, tons of enzymes, hormones, and minerals which matched the body chemistry prior to inflammation. by drinking urine, you force the body to be in inflammatory body chemistry. And support all the needed minerals and hormones. this gives you energy


You basically overwrite the body inflammatory response. Like the body says too much inflammation I will go into Th2 balance. and stay put. And you say, no way , there is not enough inflammation, go and stay more inflamed, there is all the hormones and minerals you need to do this. this is how it works for cancer.

Since in cancer body gets chronic infection and then gets inflamed trying to fight it. get to the point of inflammation where the body has to shut down the immune system and boom arginase gets induced and you have TONS of urea being produced.

Drinking urea only , will inhibit arginase, and this will keep immune system active. but it will have to retain all the minerals which were lost because of arginase inhibiting, and it is hard to guess which those were.


|But yes, I think drinking urea also has the ability to kill cancer. But it has slightly different metabolic effects.






Drinking urea usually causes fatique. but I guess it depends on the body chemistry

It would be interesting to see the difference for other people..

I have chemical urea and used to many times.( it caused fatigue in me personally , as oppose to urine causing crazy energy)


It would be great if people try urea supplements. I think urea is good on certain body chemistry, especially in those where the body is stuck in antinflammatory state.

Ornithine will do the same though.


Urea is usually good for the skin


And NOW the most important part of the puzzle. we have sick people with low and high UREA levels in the body. This is why URINE is said to balance th1 th2 ratio. Not just go into one side or another.


Since in th1 dominance you have low urea into the urine, drinking urea is bad for you in my opinion in this body chemistry


Urine is your body chemistry, you can be th1 or th2 balanced. and drinking urine puts this ratio in the middle. This is important to understand.


This is a HUGE Difference between urine and urea in this respect.


Plus you cant never overload on urine, on urea you can go from one side to another very fast. On urine you cant do that, since your urine components will change as the body chemistry keeps moving.

So urine is like a balancing things. it always tries to overwrite you into the middle of the immune system. Separate supplements cant do this, and you can swing from one side to another as people do with all the supplements.

Like they take zinc and they feel good, and then in a month the swing into the shit territory. Same exact thing with all the supplements. This is the reason I try to support everything in electrolytes protocol


I hope I made it clear of the difference between some urine components and urine. URine is dynamic and the components you will feed are not. Plus there are 2 cases of people with high and low urea in the urine. And for one case urea is good to drink and for another case not.

It is all about th1 and th2 balance, and surprisingly urine is amazing in this respect and you cant overload on it, since when you go on another side of th1 th2 balance, your urea in your urine will fall and be low. and you will be drinking urine already with very low urea and if your happen to swing into more of Th2 balance, your urea in your urine will be high and you will be drinking urine with high urea .

So it is like an automatic regulation not just of the levels of urea you are drinking but 2000 other components similar to urea which got effected by the immune system change.

Helen do you know of the symptom of never catching colds or gettig viral infections is th1 or 2? Ever since pssd I don’t EVER get sick. Definitely unbalanced but I can’t figure which way.

 

[Goose12]

Helen do you know of the symptom of never catching colds or gettig viral infections is th1 or 2? Ever since pssd I don’t EVER get sick. Definitely unbalanced but I can’t figure which way.

None of us get sick until we start to get better. I think its because we are so out of balance our bodies don't try to fight off infections even though we are loaded with bacterial and viral infections.

Getting a cold during recovery is usually a good sign. I know I felt like I had the flu after my 2nd cyle of tei.

Tei, at this point has cured my fatigue. I have steady energy all day.