PRP was prepared by collecting 20 cc of fresh blood in sodium citrate containing vaccutainers in minor operation theatre under proper aseptic precaution. The tubes were rotated in a centrifugation machine at 1500 revolutions per minute for 6 minutes. The first centrifugation is called “soft spin”, which allows blood separation into three layers, namely bottom RBC layer (55% of total volume), topmost acellular plasma layer called platelet poor plasma (PPP, 40% of total volume) and an intermediate PRP layer (5% of total volume) called the “buffy coat”. Separated buffy coat with PPP was collected with the help of Finn pipette in another test tube. This tube underwent a second centrifugation, which was longer and faster than the first, called “hard spin”, comprising at 2500 revolution per minute for 15 minutes. This allows the platelets (PRP) to settle at the bottom of the tube. The upper layer containing PPP was discarded and the lower layer of PRP was loaded in an insulin syringe containing calcium chloride (1 part calcium chloride and 9 parts of PRP) as an activator.